mouse anti human cd45 percp  (Bio-Rad)

Journal: Cell Reports Medicine

Article Title: Targeting mitochondrial complex I of CD177 + neutrophils alleviates lung ischemia-reperfusion injury

doi: 10.1016/j.xcrm.2025.102140

Figure Lengend Snippet: Enriched Cd177 + neutrophils are key contributors to lung ischemia-reperfusion injury in the mouse model (A) Nucleic acid staining and watershed-based segmentation for single-cell analysis. A representative image shows nuclei segmented using the watershed algorithm for spatial distribution analysis ( n = 2). (B) Spatial expression of the pan-immune marker Cd45 in lung tissue sections reveals regions of local immune cell infiltration. (C) Spatial mapping of Cd177 expression in lung tissues after IRI. Higher-magnification images (right) show colocalization of Cd177 with the inflammatory genes Pglyrp1 and Ltf . (D) Uniform manifold approximation and projection (UMAP) visualization showing Ly6g + Cd177 + neutrophil populations (red) compared with Ly6g + Cd177 − neutrophils (blue). The bubble heatmap on the right demonstrates enrichment of inflammatory Gene Ontology terms in Ly6g + Cd177 + cells. (E) Representative immunofluorescence images from the mouse left lung for Ly6G (green), CD177 (red), and citrullinated histone H3 (Cit-H3, white) showing NET formation in CD177 + neutrophils. Scale bar: 20 μm. (F) Reactive oxygen species (ROS) production was higher in CD177 + compared to CD177 − neutrophils from human samples (left) and in Cd177 flox/flox neutrophils compared to Cd177 flox/flox ; Ly6g Cre neutrophils from mice (right), with or without PMA stimulation. ( n = 5 per group). Neut, neutrophils; UT, untreated. (G) Quantification of MPO-DNA complexes showing increased NET formation in CD177 + compared to CD177 − human neutrophils (left) and in Cd177 flox/flox compared to Cd17 7 flox/flox ; Ly6g Cre mouse neutrophils (right) under PMA stimulation. Neut, neutrophils; UT, untreated. ( n = 5 per group). (H) Representative H&E staining of lung sections from Cd177 flox/flo x and Cd177 flox/flox ; Ly6g Cre mice under sham and IRI conditions. Quantification of acute lung injury scores (right) demonstrates significant injury in Cd177 flox/flox mice and minimal injury in Cd177 flox/flox ; Ly6g Cre mice after lung IRI. Scale bar: 50 μm. ( n = 6 per group). (I) Immunofluorescence staining revealing reduced NET infiltration (Ly6G, Cit-H3, and DNA/H1) in lung tissues of Cd177 flox/flox ; Ly6g Cre mice post-lung IRI. Scale bar: 20 μm. Data are shown as mean ± SD. Statistical significance was assessed by a two-sided Wilcoxon test adjusted with the Bonferroni method in (F), (G), and (H) and Fisher’s exact test in (D). ns, not significant; ∗∗∗ p < 0.001.

Article Snippet: PerCP-Cyanine5.5 anti-mouse/human CD45 Antibody , ThermoFisher , Cat#45-0451-82; RRID: AB_1107002.

Techniques: Staining, Single-cell Analysis, Expressing, Marker, Immunofluorescence

Journal: bioRxiv

Article Title: Exploring the Relationship Between Extracellular Vesicles, the Dendritic Cell Immunoreceptor and MicroRNA-155 in an In Vivo Model of HIV-1 Infection to Understand the Disease and Develop New Treatments

doi: 10.1101/2024.10.18.619157

Figure Lengend Snippet: Humanized NSG mice were infected with the NL4.3Balenv + EV-miR-155 viral preparations. Three groups of mice received the viral preparation: an infected + hydroxyethylcellulose group as the group of reference (n = 7), and two groups were inoculated before infection with DCIR inhibitor (n = 4) or maraviroc (n = 3). Non-infected mice were controls (n=4). A. Timeline of mice experiments (DPI: days post-infection). B. Engraftment of hematopoietic stem cells in mice was measured in blood samples. The proportion of human CD45+ cells was determined by flow cytometry. C. Proportion of CD4+ cells among T cells were assessed by flow cytometry. D. The proportion of CD8+ cells among T cells was assessed by flow cytometry. E. Peripheral blood CD4/CD8 ratio after infection calculated with flow cytometry data. F. Plasma viral load was measured at various time points. Data presented are mean with standard error of the mean (SEM). Statistical analysis was carried out by two-way ANOVA to compare the different mice subgroups (* p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001). The mouse image was obtained via BioRender.

Article Snippet: The mouse anti-human CD45-PerCP-Cy5.5 (clone 2D1), CD3-FITC (clone SK7), CD33-PE-Cy7 (clone WM-53), CD8-APC (clone HIT8a), HLA-DR-PE-Cy7 (clone L243) and CD19-APC-eFluor780 (clone SJ25C1) were purchased from Invitrogen.

Techniques: Infection, Flow Cytometry

Journal: Scientific Reports

Article Title: Effects of different conditioning regimens on HLA-mismatched microtransplantation and changes in fine immune indices in acute myeloid leukaemia

doi: 10.1038/s41598-024-70332-7

Figure Lengend Snippet: Experimental reagents.

Article Snippet: PerCP-labelled Mouse Anti-Human CD45 Antibody , BD Bioscience, USA , 662965.

Techniques: